Mutants 1 and 2 are mutations in rIIA: both are point mutations. Mutants 3 and 4 are two overlapping mutations in rIIB: 3 is a deletion mutation and 4 is a point mutation. Mutant 5 is a deletion that overlaps mutants 1 and 3 but does not overlap mutants 2 and 4.
Predict the results for the scenarios below. Select "Equal Number" if the E. coli B and E. coli K plates will have some plaques in equal proportions; select "No Plaques" if both plates will show 0 plaques; select "Can’t tell" if the information provided is insufficient to predict the result with certainty.
a. you perform a high m.o.i infection of mutants 1 and 3 on E. coli strain B. Then you harvest the lysate and perform a low m.o.i infection on E. coli strain K. Finally, you dilute this second lysate and perform low m.o.i infections on separate plates with E. coli B and E. coli K.
b. you perform a high m.o.i infection of mutants 3 and 4 on E. coli strain B. Then you harvest the lysate and perform a low m.o.i infection on E. coli strain K. Finally, you dilute this second lysate and perform low m.o.i infections on separate plates with E. coli B and E. coli K.
c. In another experiment, you perform a low m.o.i infection of mutants 4 and 5 on E. coli strain B. Then you harvest the lysate and perform a high m.o.i infection on E. coli strain K. Finally, you dilute this second lysate and perform low m.o.i infections on separate E. coli B and E. coli K plates.
d. In another experiment, you perform a low m.o.i infection of mutants 1 and 3 on E. coli strain B. Then you harvest the lysate and perform a high m.o.i infection on E. coli strain K. Finally, you dilute this second lysate and perform low m.o.i infections on separate E. coli B and E. coli K plates.