Answer:
- Substrate-level phosphorylation is a reaction process that leads to the production of adenosine triphosphate (ATP) or guanosine triphosphate (GTP) by adding high energy substrate or phosphorylated intermediate from a substrate to ADP or GDP.
- Substrate level phosphorylation can occur in the cytoplasm and/or the mitochondrial matrix during glycolysis or Krebs cycle respectively.
- Example in glycolysis
From phosphoenol Pyruvate (PEP) to Pyruvate, PEP here is a substrate where the phosphate group can be added to ADP to form ATP catalyzed by an enzyme.
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