Respuesta :
Answer: you’re gonna drag the little purple bottle looking things at the top to the vials down below on the red line
the order is met-lys-gly-gln-arg-leu-tyr-arg-ala-lle-
and that’s all u need until u can stop and move onto the next slide
it’s a little confusing but this is 100% right and the reason you don’t see all the chemical examples i showed above at first is bc after every chemical u use they give u a new set
Explanation:
During the translation phase of protein synthesis, the ribosome reads mRNA from 5' to 3' direction, while tRNA adds the correct amino acid to the protein. To do so, tRNA anticodons recognize mRNA codons and pair them.
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While the ribosome reads mRNA in the 5´ to 3´ direction, tRNA transfers the correct amino acids to build the polypeptide chain according to the codons that are being readen in the mRNA.
A codon is a short sequence of three nucleotides that store the genetic information for the aminoacids´ assembly.
Each tRNA has two important sites. One of them that couples with the codon of the mRNA molecule, named anticodon. The other site couples with an amino acid.
tRNA allows amino acids to align according to the nucleotidic sequence in the mRNA molecule.
When the new amino acid binds the growing peptidic chain, the tRNA molecule leaves the ribosome, leaving a space for the next tRNA.
• The start codon AUG is the most common sequence used by eukaryotic cells and places near the 5´extreme of the molecule.
• The end codons are UAA, UAG, UGA.
In the exposed example, we have the following mRNA sequence:
(Note: I suggest you check if your sequence is similar to this one. If not, just follow the steps and do the same procedure with your sequence).
5' - AUGAAGGGCCAACGACUUUAU-3'
Now, we will separate this sequence into codons, leaving a space between them. There are seven codons.
5' - AUG AAG GGC CAA CGA CUU UAU-3'
Now, we need to define which are the tRNA anticodons that recognize and pair these codons. To do it, remember that,
- Adenine pairs Uracil
- Guanine pairs Cytokine
mRNA codons 5' - AUG AAG GGC CAA CGA CUU UAU-3'
tRNA antidocons UAC UUC CCG GUU GCU GAA AUA
So, as the ribosome moves along the mRNA, you need to place each of the tRNA carring these anticodons in the free space. Each anticodon needs to pair its corresponding codon. Remember to start with the anticodon UAC.
Now, let us see which amino acids are being added to the protein. Remember that the amino acid is defined by the codons, not the anticodons.
mRNA codons 5' - AUG AAG GGC CAA CGA CUU UAU-3'
tRNA antidocons UAC UUC CCG GUU GCU GAA AUA
amino acids Met Lys Gly Gln Arg Leu Tyr
Finally, the protein is as follows: Met-Lys-Gly-Gln-Arg-Leu-Tyr
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