The reaction rate is affected if the vitamin C is added in lower quantity due to lower amount of reactants.
If you added 40.0 mL of Vitamin C stock solution instead of 25.0 mL when preparing Solution, the reaction rate is affected because of the lower amount of reactant for the chemical reaction. If the reactants are present in larger amount then the products are also produced in large concentration.
So we can conclude that the reaction rate is affected if the vitamin C is added in lower quantity due to lower amount of reactants.
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