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examine the entire genome of a bacteria and notice that nearly 70% of all DNA base pairs have three hydrogen bonds. This is a clear example of ____________________.

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Examine the entire genome of a bacteria and notice that nearly 70% of all DNA base pairs have three hydrogen bonds. This is a clear example of GC content bias.

What is GC Content Bias?

  • In Illumina sequencing data, the relationship between fragment count (read coverage) and GC content is known as "GC content bias."
  • For techniques that concentrate on determining the number of fragments within a genome, like copy number estimation, this bias may predominate the signal of interest (DNA-sequence).
  • The bias varies between samples, and there is no agreement on the most effective ways to eliminate it in a particular sample.
  • High-throughput Illumina Genome Analyzer sequencing has gained popularity as a method for assessing the relative amount of DNA in an assay.
  • The DNA of interest is broken up using this technique, and either the ends of the fragments are sequenced. These read pairs, which are short sequences, are mapped to a reference genome.

To learn more about GC Content Bias refer to:

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